DegronMD

Uniprot ID: Q13153

Source protein: Serine/threonine-protein kinase PAK 1

Source gene: PAK1

Class: DEG_SCF_FBW7_1

Location: 414:419

E3 ligase: FBXW7

DEGRON score: 0.5946

ASA_SCORE: 73.1333

COIL: 0.7625

CONS_SCORE: 0.4587

DSS_SCORE: 0.3268

HELIX: 0.1252

FCONS_SCORE: 0.6148

RIG_SCORE: 0.7934

Degron related mutations and impact score

Mutation	Sample	Cancer	Dataset	Degron	Degron class	Impact score
p.R421L	TCGA-AA-3947	COAD	TCGA	ITPEQS	DEG_SCF_FBW7_1	0.2778
p.R421L	DO8192	COAD	ICGC	ITPEQS	DEG_SCF_FBW7_1	0.2778
p.F410L	TCGA-AZ-4315	COAD	TCGA	ITPEQS	DEG_SCF_FBW7_1	0.2222
p.F410L	DO8842	COAD	ICGC	ITPEQS	DEG_SCF_FBW7_1	0.2222
p.F410L	SIDM00217	Colorectal Carcinoma	GDSC	ITPEQS	DEG_SCF_FBW7_1	0.2222
p.F410L	ACH-000999	Colon/Colorectal Cancer	CCLE	ITPEQS	DEG_SCF_FBW7_1	0.2222
p.F410L	SNU-1040	large_intestine	COSMIC	ITPEQS	DEG_SCF_FBW7_1	0.2222
p.L405I	TCGA-CV-A6JM	HNSC	TCGA	ITPEQS	DEG_SCF_FBW7_1	0.0833
p.L405I	DO49991	HNSC	ICGC	ITPEQS	DEG_SCF_FBW7_1	0.0833
p.E417Q	TCGA-G2-AA3B	BLCA	TCGA	ITPEQS	DEG_SCF_FBW7_1	0.5633
p.E417Q	DO51882	BLCA	ICGC	ITPEQS	DEG_SCF_FBW7_1	0.5633
p.T406N	DO228971	LICA	ICGC	ITPEQS	DEG_SCF_FBW7_1	0.1111
p.T415N	DO228985	LICA	ICGC	ITPEQS	DEG_SCF_FBW7_1	0.8166
p.P416L	DO229494	SKCA	ICGC	ITPEQS	DEG_SCF_FBW7_1	0.7582
p.T406A	SIDM01045	Endometrial Carcinoma	GDSC	ITPEQS	DEG_SCF_FBW7_1	0.1111
p.T406A	EN	endometrium	COSMIC	ITPEQS	DEG_SCF_FBW7_1	0.1111
p.D407G	MFE-319	endometrium	COSMIC	ITPEQS	DEG_SCF_FBW7_1	0.1389
p.V420L	H3255	lung	COSMIC	ITPEQS	DEG_SCF_FBW7_1	0.75

Drug responses associated with PAK1 mutations

Sample	Cancer	Mutation (In Degron?)	Dataset	Drug	IC50
SIDM00227	NB\|neuroblastoma	p.S259N (No)	GDSC	IPA-3	600.2589
SIDM00227	NB\|neuroblastoma	p.S259N (No)	GDSC	PAK_5339	20.887
SIDM01034	UNCLASSIFIED\|B_cell_leukemia	p.S382L (No)	GDSC	IPA-3	4.7953
SIDM01034	UNCLASSIFIED\|B_cell_leukemia	p.S382L (No)	GDSC	PAK_5339	3.1572
SIDM01007	ALL\|lymphoblastic_leukemia	p.R371C (No)	GDSC	IPA-3	7.9125
SIDM01007	ALL\|lymphoblastic_leukemia	p.R371C (No)	GDSC	PAK_5339	4.5277
SIDM00704	SCLC\|lung_small_cell_carcinoma	p.K11N (No)	GDSC	IPA-3	15.6262
SIDM00704	SCLC\|lung_small_cell_carcinoma	p.K11N (No)	GDSC	PAK_5339	2.2298
SIDM01045	UCEC\|endometrium	p.T406A (Yes)	GDSC	IPA-3	799.4468
SIDM01045	UCEC\|endometrium	p.T406A (Yes)	GDSC	PAK_5339	4.1583
SIDM00195	UNCLASSIFIED\|ewings_sarcoma	p.M531I (No)	GDSC	IPA-3	58.1219
SIDM00195	UNCLASSIFIED\|ewings_sarcoma	p.M531I (No)	GDSC	PAK_5339	13.406
SIDM00333	UCEC\|endometrium	p.D505G (No)	GDSC	IPA-3	28.4161
SIDM00333	UCEC\|endometrium	p.D505G (No)	GDSC	PAK_5339	9.5851
SIDM00217	COREAD\|large_intestine	p.I452T (No)	GDSC	PAK_5339	21.466
SIDM00217	COREAD\|large_intestine	p.F410L (Yes)	GDSC	PAK_5339	21.466
SIDM00217	COREAD\|large_intestine	p.A163V (No)	GDSC	PAK_5339	21.466
SIDM00955	SKCM\|melanoma	p.P73L (No)	GDSC	IPA-3	139.3185
SIDM00534	COREAD\|large_intestine	p.N111H (No)	GDSC	IPA-3	249.9345
SIDM00534	COREAD\|large_intestine	p.N111H (No)	GDSC	PAK_5339	13.792
SIDM01154	ALL\|lymphoblastic_T_cell_leukaemia	p.V403A (Yes)	GDSC	IPA-3	38.771
SIDM01154	ALL\|lymphoblastic_T_cell_leukaemia	p.V403A (Yes)	GDSC	PAK_5339	5.703
SIDM00927	SCLC\|lung_small_cell_carcinoma	p.G364V (No)	GDSC	IPA-3	119.1757
SIDM00046	LUAD\|lung_NSCLC_adenocarcinoma	p.V518L (No)	GDSC	IPA-3	112.8572
SIDM00046	LUAD\|lung_NSCLC_adenocarcinoma	p.V518L (No)	GDSC	PAK_5339	11.735
SIDM00746	LUAD\|lung_NSCLC_adenocarcinoma	p.N4S (No)	GDSC	IPA-3	406.8579
SIDM00193	COREAD\|large_intestine	p.R53Q (No)	GDSC	PAK_5339	17.375
SIDM00118	COREAD\|large_intestine	p.V445F (No)	GDSC	IPA-3	38.6275
SIDM00118	COREAD\|large_intestine	p.V445F (No)	GDSC	PAK_5339	9.2108
SIDM00118	COREAD\|large_intestine	p.I312S (No)	GDSC	IPA-3	38.6275
SIDM00118	COREAD\|large_intestine	p.I312S (No)	GDSC	PAK_5339	9.2108

DegronMD: protein-targeted degradation, mutation and drug response to degrons

Degron: ITPEQS

Degron related mutations and impact score

Drug responses associated with PAK1 mutations

All proteins holding degron of ITPEQS

Uniprot ID	Source protein	Degron class	Location	Hit	E3 ligase	Degron score	Detail
O75914	Serine/threonine-protein kinase PAK 3	DEG_SCF_FBW7_1	427:432	ITPEQS	FBXW7	0.5946	More...
Q13153	Serine/threonine-protein kinase PAK 1	DEG_SCF_FBW7_1	414:419	ITPEQS	FBXW7	0.5946	More...