| Tag | Content |
|---|---|
| Uniprot ID | Q63014 |
| Entrez ID | 116633 |
| Genbank protein ID | AAA95941.1 |
| Genbank nucleotide ID | NM_053855.1 |
| Ensembl protein ID | ENSRNOP00000008827 |
| Ensembl nucleotide ID | ENSRNOG00000006559 |
| Gene name | A-kinase anchor protein 8 |
| Gene symbol | Akap8 |
| Organism | Rattus norvegicus |
| NCBI taxa ID | 10116 |
| Cleft type | |
| Developmental stage | |
| Data sources | Homology search |
| Reference | |
| Functional description | Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II) (PubMed:8125992). Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring. May help to deliver cyclin D/E to CDK4 to facilitate cell cycle progression. Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function may act redundantly with AKAP8L. Involved in nuclear retention of RPS6KA1 upon ERK activation thus inducing cell proliferation. May be involved in regulation of DNA replication by acting as scaffold for MCM2. Enhances HMT activity of the KMT2 family MLL4/WBP7 complex and is involved in transcriptional regulation. In a teratocarcinoma cell line is involved in retinoic acid-mediated induction of developmental genes implicating H3 'Lys-4' methylation. May be involved in recruitment of active CASP3 to the nucleus in apoptotic cells. May act as a carrier protein of GJA1 for its transport to the nucleus. Seems to involved in modulation of rDNA transcription. Preferentially binds GC-rich DNA in vitro and associates to GC-rich ribosomal RNA promoters. Involved in modulation of Toll-like receptor signaling. Required for the cAMP-dependent suppression of TNF-alpha in early stages of LPS-induced macrophage activation; the function probably implicates targeting of PKA to NFKB1 (By similarity). |
| Sequence | MEQSYGGYGA WSAGPANTQG TYGSGVASWQ GYENYSYYNA QNTSVPTGTP YSYGPASWEA 60 TKASDGGLAA GSSAMHVASF APEPCTDNSD SLIAKINQRL DMLSKEGGRG GISSGGEGMQ 120 DRDSSFRFQP YESYDSRPCM PEHTPYRPSY SYDYDFDLGT DRNGSFGGTF NDCRDPTPER 180 GALDGFLRGR GQGRFQDRSN SSTFIRSDPF MPPSASSEPL STTWSELNYM GGRGLGGPST 240 NRPPPSLFSQ SMAPDYSMMG MQGVGGFGGT MPYGCGRSQT RIRDWPRRRG FERFGPDNMG 300 RKRKPFPLYE EPDAKLARAD SEGDLSENDD GAGDLRSGDE EFRGEDDLCD SRKQRGEKED 360 EDEDVKKRRE KQRRRDRMRD RAADRIQFAC SVCKFRSFED EEIQKHLQSK FHKETLRFIS 420 TKLPDKTVEF LQEYIINRNK KIEKRRQELL EKESPKPKPD PFKGIGQEHF FKRIEAAHCL 480 ACDMLIPAQH QLLQRHLHSV DHNHNRRLAA EQFKKTSLHV AKSVLNNKHI VKMLEKYLKG 540 EDPFVNETAD LETEGDENLG EEKETPEEVA AEVLAEVITA AVKAVEGDGE PAAEHSDVLA 600 EVEGPVDTAE AGSDSHTGKL LEEQTCETAS ETRNMEDMAR GEAAEARNEA AVPAAAAGSP 660 VPVIAIPGIL EDELEQTDAE AKDTPTE 687 |
Abbreviation :
CLO : cleft lip only. CPO : cleft palate only.
CLP : cleft lip and palate. CL/P : cleft lip with/without cleft palate.
For humans: CL/P, CLO, CPO, and CLP. For mice: CLO, CLP, and CPO.
| Relation | Gene symbol | Entrez ID | UniProt ID | Cleft type | Developmental stage | Species | Evidence | Details |
|---|---|---|---|---|---|---|---|---|
| 1:1 ortholog | AKAP8 | A0A3Q1M9C8 | Bos taurus | Prediction | More>> | |||
| 1:1 ortholog | AKAP8 | A0A452DXJ3 | Capra hircus | Prediction | More>> | |||
| 1:1 ortholog | AKAP8 | 10270 | O43823 | Homo sapiens | Prediction | More>> | ||
| 1:1 ortholog | Akap8 | 56399 | Q9DBR0 | CPO | E14.5 | Mus musculus | Publication | More>> |
| 1:1 ortholog | AKAP8 | 748028 | K7D8I9 | Pan troglodytes | Prediction | More>> | ||
| 1:1 ortholog | Akap8 | 116633 | Q63014 | Rattus norvegicus | Prediction | More>> |
| GO ID | GO Term |
|---|---|
| GO:0000793 | condensed chromosome |
| GO:0001939 | female pronucleus |
| GO:0005794 | Golgi apparatus |
| GO:0005739 | mitochondrion |
| GO:0016363 | nuclear matrix |
| GO:0005730 | nucleolus |
| GO:0005654 | nucleoplasm |
| GO:0005634 | nucleus |
| GO:0003677 | DNA binding |
| GO:0003690 | double-stranded DNA binding |
| GO:0042826 | histone deacetylase binding |
| GO:0051059 | NF-kappaB binding |
| GO:0034237 | protein kinase A regulatory subunit binding |
| GO:0008270 | zinc ion binding |
| GO:0044839 | cell cycle G2/M phase transition |
| GO:0071222 | cellular response to lipopolysaccharide |
| GO:0071380 | cellular response to prostaglandin E stimulus |
| GO:0045087 | innate immune response |
| GO:0007076 | mitotic chromosome condensation |
| GO:0032720 | negative regulation of tumor necrosis factor production |
| GO:0031065 | positive regulation of histone deacetylation |
| GO:0015031 | protein transport |
| GO:0033127 | regulation of histone phosphorylation |
| Keyword ID | Keyword Term |
|---|---|
| KW-0963 | Cytoplasm |
| KW-0238 | DNA-binding |
| KW-0391 | Immunity |
| KW-0399 | Innate immunity |
| KW-1017 | Isopeptide bond |
| KW-0479 | Metal-binding |
| KW-0488 | Methylation |
| KW-0539 | Nucleus |
| KW-0597 | Phosphoprotein |
| KW-0653 | Protein transport |
| KW-1185 | Reference proteome |
| KW-0677 | Repeat |
| KW-0804 | Transcription |
| KW-0805 | Transcription regulation |
| KW-0813 | Transport |
| KW-0832 | Ubl conjugation |
| KW-0862 | Zinc |
| KW-0863 | Zinc-finger |
| PROSITE ID | PROSITE Term |
|---|---|
| PS51799 | ZF_C2H2_AKAP95 |
| Pfam ID | Pfam Term |
|---|---|
| PF04988 | AKAP95 |