| Tag | Content |
|---|---|
| Uniprot ID | Q9DBR0; Q9R0L8; |
| Entrez ID | 56399 |
| Genbank protein ID | BAA84710.1; BAB23574.1; BAC40746.1; |
| Genbank nucleotide ID | NM_019774.5 |
| Ensembl protein ID | ENSMUSP00000002699 |
| Ensembl nucleotide ID | ENSMUSG00000024045 |
| Gene name | A-kinase anchor protein 8 |
| Gene symbol | Akap8 |
| Organism | Mus musculus |
| NCBI taxa ID | 10090 |
| Cleft type | CPO |
| Developmental stage | E14.5 |
| Data sources | Manually collected |
| Reference | 16751186 |
| Functional description | Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II). Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring (By similarity). Specifically involved in recruitment of CAPD2 to, and condensation of maternal but not paternal chromosomes (PubMed:12082153). May help to deliver cyclin D/E to CDK4 to facilitate cell cycle progression (PubMed:14641107). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function may act redundantly with AKAP8L. Involved in nuclear retention of RPS6KA1 upon ERK activation thus inducing cell proliferation. May be involved in regulation of DNA replication by acting as scaffold for MCM2. Enhances HMT activity of the KMT2 family MLL4/WBP7 complex and is involved in transcriptional regulation. In a teratocarcinoma cell line is involved in retinoic acid-mediated induction of developmental genes implicating H3 'Lys-4' methylation. May be involved in recruitment of active CASP3 to the nucleus in apoptotic cells. May act as a carrier protein of GJA1 for its transport to the nucleus. Seems to involved in modulation of rDNA transcription. Preferentially binds GC-rich DNA in vitro and associates to GC-rich ribosomal RNA promoters (By similarity). Involved in modulation of Toll-like receptor signaling. Required for the cAMP-dependent suppression of TNF-alpha in early stages of LPS-induced macrophage activation; the function probably implicates targeting of PKA to NFKB1 (PubMed:19531803). |
| Sequence | MEQGYGGYGA WSAGPANTQG TYGSGMTSWQ GYENYNYYNA QNTSVPAGTP YSYGPASWEA 60 TKTNDGGLAA GSPAMHVASF APEPCTDNSD SLIAKINQRL DMLSKEGGRG GISSGGEGVQ 120 DRDSSFRFQP YESYDARPCI PEHNPYRPGY GYDYDFDLGT DRNGSFGGTF NDCRDPAPER 180 GSLDGFLRGR GQGRFQDRSN SSTFIRSDPF MPPSASEPLS TTWNELNYMG GRGLGGPSTS 240 RPPPSLFSQS MAPDYSMMGM QGVGGFGGTM PYGCGRSQTR IRDWPRRRGF ERFGPDNMGR 300 KRKQFPLYEE PDAKLARADS DGDLSENDDG AGDLRSGDEE FRGEDDLCDS RKQRGEKEDE 360 DEDVKKRREK QRRRDRMRDR AADRIQFACS VCKFRSFEDE EIQKHLQSKF HKETLRFIST 420 KLPDKTVEFL QEYIINRNKK IEKRRQELLE KESPKPKPDP FKGIGQEHFF KKIEAAHCLA 480 CDMLIPAQHQ LLQRHLHSVD HNHNRRLAAE QFKKTSLHVA KSVLNNKHIV KMLEKYLKGE 540 DPFVNETADL ETEGDENVGE EKEETPEEVA AEVLAEVITA AVKAVEGEGE PAAAHSDVLT 600 EVEGPVDTAE ASSDPHTEKL LEEQTCEAAS ETRSIEDKTR GEAAEARNEA AMPTADAGST 660 LPVIAIPGIM EDELEQTGAE AKDIPTE 687 |
Abbreviation :
CLO : cleft lip only. CPO : cleft palate only.
CLP : cleft lip and palate. CL/P : cleft lip with/without cleft palate.
For humans: CL/P, CLO, CPO, and CLP. For mice: CLO, CLP, and CPO.
| Relation | Gene symbol | Entrez ID | UniProt ID | Cleft type | Developmental stage | Species | Evidence | Details |
|---|---|---|---|---|---|---|---|---|
| 1:1 ortholog | AKAP8 | A0A3Q1M9C8 | Bos taurus | Prediction | More>> | |||
| 1:1 ortholog | AKAP8 | A0A452DXJ3 | Capra hircus | Prediction | More>> | |||
| 1:1 ortholog | AKAP8 | 10270 | O43823 | Homo sapiens | Prediction | More>> | ||
| 1:1 ortholog | Akap8 | 56399 | Q9DBR0 | CPO | E14.5 | Mus musculus | Publication | More>> |
| 1:1 ortholog | AKAP8 | 748028 | K7D8I9 | Pan troglodytes | Prediction | More>> | ||
| 1:1 ortholog | Akap8 | 116633 | Q63014 | Rattus norvegicus | Prediction | More>> |
| GO ID | GO Term | Evidence |
|---|---|---|
| GO:0003677 | DNA binding | ISO |
| GO:0003682 | chromatin binding | ISO |
| GO:0003690 | double-stranded DNA binding | ISO |
| GO:0005515 | protein binding | IPI |
| GO:0008270 | zinc ion binding | ISO |
| GO:0034237 | protein kinase A regulatory subunit binding | ISO |
| GO:0034237 | protein kinase A regulatory subunit binding | IBA |
| GO:0042826 | histone deacetylase binding | ISO |
| GO:0051059 | NF-kappaB binding | IDA |
| GO ID | GO Term | Evidence |
|---|---|---|
| GO:0007076 | mitotic chromosome condensation | ISO |
| GO:0007076 | mitotic chromosome condensation | IDA |
| GO:0015031 | protein transport | IEA |
| GO:0031065 | positive regulation of histone deacetylation | ISO |
| GO:0032720 | negative regulation of tumor necrosis factor production | IMP |
| GO:0033127 | regulation of histone phosphorylation | ISO |
| GO:0044839 | cell cycle G2/M phase transition | ISO |
| GO:0045087 | innate immune response | IEA |
| GO:0071222 | cellular response to lipopolysaccharide | IMP |
| GO:0071380 | cellular response to prostaglandin E stimulus | IMP |
| GO ID | GO Term | Evidence |
|---|---|---|
| GO:0000790 | nuclear chromatin | ISO |
| GO:0000793 | condensed chromosome | ISO |
| GO:0000793 | condensed chromosome | IDA |
| GO:0001939 | female pronucleus | IDA |
| GO:0005634 | nucleus | IDA |
| GO:0005654 | nucleoplasm | ISO |
| GO:0005730 | nucleolus | ISO |
| GO:0005739 | mitochondrion | ISO |
| GO:0005794 | Golgi apparatus | ISO |
| GO:0016363 | nuclear matrix | ISO |
| GO:0016363 | nuclear matrix | IDA |
| GO:0016363 | nuclear matrix | IBA |
| Reactome ID | Reactome Term | Evidence |
|---|
| Keyword ID | Keyword Term |
|---|---|
| KW-0963 | Cytoplasm |
| KW-0238 | DNA-binding |
| KW-0391 | Immunity |
| KW-0399 | Innate immunity |
| KW-1017 | Isopeptide bond |
| KW-0479 | Metal-binding |
| KW-0488 | Methylation |
| KW-0539 | Nucleus |
| KW-0597 | Phosphoprotein |
| KW-0653 | Protein transport |
| KW-1185 | Reference proteome |
| KW-0677 | Repeat |
| KW-0804 | Transcription |
| KW-0805 | Transcription regulation |
| KW-0813 | Transport |
| KW-0832 | Ubl conjugation |
| KW-0862 | Zinc |
| KW-0863 | Zinc-finger |
| PROSITE ID | PROSITE Term |
|---|---|
| PS51799 | ZF_C2H2_AKAP95 |
| Pfam ID | Pfam Term |
|---|---|
| PF04988 | AKAP95 |