| Tag | Content |
|---|---|
| Uniprot ID | Q9DBG3; Q80XJ4; |
| Entrez ID | 71770 |
| Genbank protein ID | AAH46772.1; BAB23711.1; |
| Genbank nucleotide ID | NM_027915.3; NM_001035854.2; XM_017314772.1; XM_006534258.3; |
| Ensembl protein ID | ENSMUSP00000070714; ENSMUSP00000018875; |
| Ensembl nucleotide ID | ENSMUSG00000035152 |
| Gene name | AP-2 complex subunit beta |
| Gene symbol | Ap2b1 |
| Organism | Mus musculus |
| NCBI taxa ID | 10090 |
| Cleft type | CPO |
| Developmental stage | E14.5 |
| Data sources | Manually collected |
| Reference | 20500056; 25807483; |
| Functional description | Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10 (PubMed:23676497). The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins; at least some clathrin-associated sorting proteins (CLASPs) are recognized by their [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly; clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly (By similarity). |
| Sequence | MTDSKYFTTN KKGEIFELKA ELNNEKKEKR KEAVKKVIAA MTVGKDVSSL FPDVVNCMQT 60 DNLELKKLVY LYLMNYAKSQ PDMAIMAVNS FVKDCEDPNP LIRALAVRTM GCIRVDKITE 120 YLCEPLRKCL KDEDPYVRKT AAVCVAKLHD INAQMVEDQG FLDSLRDLIA DSNPMVVANA 180 VAALSEISES HPNSNLLDLN PQNINKLLTA LNECTEWGQI FILDCLSNYN PKDDREAQSI 240 CERVTPRLSH ANSAVVLSAV KVLMKFLELL PKDSDYYNML LKKLAPPLVT LLSGEPEVQY 300 VALRNINLIV QKRPEILKQE IKVFFVKYND PIYVKLEKLD IMIRLASQAN IAQVLAELKE 360 YATEVDVDFV RKAVRAIGRC AIKVEQSAER CVSTLLDLIQ TKVNYVVQEA IVVIRDIFRK 420 YPNKYESIIA TLCENLDSLD EPDARAAMIW IVGEYAERID NADELLESFL EGFHDESTQV 480 QLTLLTAIVK LFLKKPSETQ ELVQQVLSLA TQDSDNPDLR DRGYIYWRLL STDPVTAKEV 540 VLSEKPLISE ETDLIEPTLL DELICHIGSL ASVYHKPPNA FVEGSHGIHR KHLPIHHGST 600 DAGDSPVGTT TTTNLEQPQV IPSQGDLLGD LLNLDLGPPV NVPQVSSMQM GAVDLLGGGL 660 DSLVGQSFIP SSVPATFAPS PTPAVVSSGL NDLFELSTGI GMAPGGYVAP KAVWLPAVKA 720 KGLEISGTFT HRQGHIYMEM NFTNKALQHM TDFAIQFNKN SFGVIPSTPL AIHTPLMPNQ 780 SIDVSLPLNT LGPVMKMEPL NNLQVAVKNN IDVFYFSCLI PLNVLFVEDG KMERQVFLAT 840 WKDIPNENEL QFQIKECHLN ADTVSSKLQN NNVYTIAKRN VEGQDMLYQS LKLTNGIWIL 900 AELRIQPGNP NYTLSLKCRA PEVSQYIYQV YDSILKN 937 |
Abbreviation :
CLO : cleft lip only. CPO : cleft palate only.
CLP : cleft lip and palate. CL/P : cleft lip with/without cleft palate.
For humans: CL/P, CLO, CPO, and CLP. For mice: CLO, CLP, and CPO.
| Relation | Gene symbol | Entrez ID | UniProt ID | Cleft type | Developmental stage | Species | Evidence | Details |
|---|---|---|---|---|---|---|---|---|
| 1:1 ortholog | AP2B1 | 480605 | E2RIV1 | Canis lupus familiaris | Prediction | More>> | ||
| 1:1 ortholog | AP2B1 | A0A452F9H3 | Capra hircus | Prediction | More>> | |||
| 1:1 ortholog | AP2B1 | 163 | P63010 | Homo sapiens | Prediction | More>> | ||
| 1:1 ortholog | Ap2b1 | 71770 | Q9DBG3 | CPO | E14.5 | Mus musculus | Publication | More>> |
| 1:1 ortholog | AP2B1 | 454587 | K7CXZ7 | Pan troglodytes | Prediction | More>> | ||
| 1:1 ortholog | AP2B1 | A0A480V5W1 | Sus scrofa | Prediction | More>> | |||
| 1:1 ortholog | AP2B1 | 100347007 | G1SL02 | Oryctolagus cuniculus | Prediction | More>> | ||
| 1:1 ortholog | Ap2b1 | 140670 | P62944 | Rattus norvegicus | Prediction | More>> | ||
| 1:1 ortholog | ap2b1 | E9QC84 | Danio rerio | Prediction | More>> |
| GO ID | GO Term | Evidence |
|---|---|---|
| GO:0005515 | protein binding | IPI |
| GO:0030276 | clathrin binding | ISO |
| GO:0044877 | protein-containing complex binding | IEA |
| GO ID | GO Term | Evidence |
|---|---|---|
| GO:0003279 | cardiac septum development | IMP |
| GO:0003281 | ventricular septum development | IMP |
| GO:0006886 | intracellular protein transport | IEA |
| GO:0007507 | heart development | IMP |
| GO:0016192 | vesicle-mediated transport | IBA |
| GO:0035904 | aorta development | IMP |
| GO:0045807 | positive regulation of endocytosis | IEA |
| GO:0048268 | clathrin coat assembly | IEA |
| GO:0060976 | coronary vasculature development | IMP |
| GO:0072583 | clathrin-dependent endocytosis | IDA |
| GO:0098884 | postsynaptic neurotransmitter receptor internalization | IDA |
| GO:0098884 | postsynaptic neurotransmitter receptor internalization | IMP |
| GO:0099590 | neurotransmitter receptor internalization | IMP |
| GO:1901215 | negative regulation of neuron death | IEA |
| GO:1905477 | positive regulation of protein localization to membrane | IEA |
| GO ID | GO Term | Evidence |
|---|---|---|
| GO:0030122 | AP-2 adaptor complex | IDA |
| GO:0030131 | clathrin adaptor complex | ISO |
| GO:0098794 | postsynapse | IEA |
| GO:0098978 | glutamatergic synapse | IDA |
| GO:0098978 | glutamatergic synapse | IMP |
| Reactome ID | Reactome Term | Evidence |
|---|
| InterPro ID | InterPro Term |
|---|---|
| IPR026739 | AP_beta |
| IPR016342 | AP_complex_bsu_1_2_4 |
| IPR011989 | ARM-like |
| IPR016024 | ARM-type_fold |
| IPR000225 | Armadillo |
| IPR015151 | B-adaptin_app_sub_C |
| IPR002553 | Clathrin/coatomer_adapt-like_N |
| IPR008152 | Clathrin_a/b/g-adaptin_app_Ig |
| IPR013041 | Clathrin_app_Ig-like_sf |
| IPR013037 | Clathrin_b-adaptin_app_Ig-like |
| IPR009028 | Coatomer/calthrin_app_sub_C |
| IPR012295 | TBP_dom_sf |