| Tag | Content |
|---|---|
| Uniprot ID | A2A935; Q69ZD6; Q6PB79; Q7TPF4; |
| Entrez ID | 70673 |
| Genbank protein ID | BAC79382.1; AAH59838.1; BAD32508.1; |
| Genbank nucleotide ID | XM_006539173.3; NM_027504.3; NM_001291026.1; NM_001291029.1; |
| Ensembl protein ID | ENSMUSP00000064546; ENSMUSP00000030902; |
| Ensembl nucleotide ID | ENSMUSG00000039410 |
| Gene name | Histone-lysine N-methyltransferase PRDM16 |
| Gene symbol | Prdm16 |
| Organism | Mus musculus |
| NCBI taxa ID | 10090 |
| Cleft type | CPO |
| Developmental stage | E14.5 |
| Data sources | Manually collected |
| Reference | 32044379; 20007998; 11818962; |
| Functional description | Binds DNA and functions as a transcriptional regulator (PubMed:18483224). Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro (PubMed:22939622). Probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation (PubMed:22939622). Likely to be one of the primary histone methyltransferases along with MECOM/PRDM3 that direct cytoplasmic H3K9me1 methylation (PubMed:22939622). Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism (PubMed:17618855, PubMed:18483224). Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells (PubMed:18719582, PubMed:19641492). Functions as a repressor of TGF-beta signaling. |
| Sequence | MRSKARARKL AKSDGDVVNN MYEPDPDLLA GQSAEEETED GILSPIPMGP PSPFPTSEDF 60 TPKEGSPYEA PVYIPEDIPI PPDFELRESS IPGAGLGIWA KRKMEIGERF GPYVVTPRAA 120 LKEADFGWEM LTDTEVSSQE SCIKKQISED LGSEKFCVDA NQAGSGSWLK YIRVACSCDD 180 QNLAMCQINE QIYYKVIKDI EPGEELLVHV KEGAYSLGVM APSLDEDPTF RCDECDELFQ 240 CRLDLRRHKK YACSSAGAQL YEGLGEELKP EGLGVGSDGQ AHECKDCERM FPNKYSLEQH 300 MIVHTEEREY KCDQCPKAFN WKSNLIRHQM SHDSGKRFEC ENCVKVFTDP SNLQRHIRSQ 360 HVGARAHACP DCGKTFATSS GLKQHKHIHS TVKPFICEVC HKSYTQFSNL CRHKRMHADC 420 RTQIKCKDCG QMFSTTSSLN KHRRFCEGKN HYTPGSIFTP GLPLTPSPMM DKTKPSPTLN 480 HGGLGFSEYF PSRPHPGSLP FSAAPPAFPA LTPGFPGIFP PSLYPRPPLL PPTPLLKSPL 540 NHAQDAKLPS PLGNPALPLV SAVSNSSQGA TAATGSEEKF DGRLEDAYAE KVKNRSPDMS 600 DGSDFEDINT TTGTDLDTTT GTGSDLDSDL DSDRDKGKDK GKPVESKPEF GGASVPPGAM 660 NSVAEVPAFY SQHSFFPPPE EQLLTASGAA GDSIKAIASI AEKYFGPGFM SMQEKKLGSL 720 PYHSVFPFQF LPNFPHSLYP FTDRALAHNL LVKAEPKSPR DALKVGGPSA ECPFDLTTKP 780 KEAKPALLAP KVPLIPSSGE EQPLDLSIGS RARASQNGGG REPRKNHVYG ERKPGVSEGL 840 PKVCPAQLPQ QPSLHYAKPS PFFMDPIYRV EKRKVADPVG VLKEKYLRPS PLLFHPQMSA 900 IETMTEKLES FAAMKADSGS SLQPLPHHPF NFRSPPPTLS DPILRKGKER YTCRYCGKIF 960 PRSANLTRHL RTHTGEQPYR CKYCDRSFSI SSNLQRHVRN IHNKEKPFKC HLCNRCFGQQ 1020 TNLDRHLKKH EHEGAPVSQH SGVLTNHLGT SASSPTSESD NHALLDEKED SYFSEIRNFI 1080 ANSEMNQAST RMDKRPEIQD LDSNPPCPGS ASAKPEDVEE EEEEELEEED DDSLAGKSQE 1140 DTVSPTPEPQ GVYEDEEDEE PPSLTMGFDH TRRCVEERGG GLLALEPTPT FGKGLDLRRA 1200 AEEAFEVKDV LNSTLDSEVL KQTLYRQAKN QAYAMMLSLS EDTPLHAPSQ SSLDAWLNIT 1260 GPSSESGAFN PINHL 1275 |
Abbreviation :
CLO : cleft lip only. CPO : cleft palate only.
CLP : cleft lip and palate. CL/P : cleft lip with/without cleft palate.
For humans: CL/P, CLO, CPO, and CLP. For mice: CLO, CLP, and CPO.
| Relation | Gene symbol | Entrez ID | UniProt ID | Cleft type | Developmental stage | Species | Evidence | Details |
|---|---|---|---|---|---|---|---|---|
| 1:1 ortholog | PRDM16 | A0A452F1P6 | Capra hircus | Prediction | More>> | |||
| 1:1 ortholog | PRDM16 | 63976 | Q9HAZ2 | Homo sapiens | Prediction | More>> | ||
| 1:1 ortholog | Prdm16 | 70673 | A2A935 | CPO | E14.5 | Mus musculus | Publication | More>> |
| 1:1 ortholog | PRDM16 | 457227 | H2R9K2 | Pan troglodytes | Prediction | More>> | ||
| 1:1 ortholog | Prdm16 | 100366024 | A0A096MJ70 | Rattus norvegicus | Prediction | More>> | ||
| 1:1 ortholog | prdm16 | B8JHX5 | Danio rerio | Prediction | More>> |
| GO ID | GO Term | Evidence |
|---|---|---|
| GO:0003713 | transcription coactivator activity | IDA |
| GO:0005515 | protein binding | IPI |
| GO:0033613 | activating transcription factor binding | ISO |
| GO:0043565 | sequence-specific DNA binding | ISO |
| GO:0043565 | sequence-specific DNA binding | IDA |
| GO:0046332 | SMAD binding | IPI |
| GO:0046872 | metal ion binding | IEA |
| GO:0046974 | histone methyltransferase activity (H3-K9 specific) | IDA |
| GO ID | GO Term | Evidence |
|---|---|---|
| GO:0000122 | negative regulation of transcription by RNA polymerase II | ISS |
| GO:0000122 | negative regulation of transcription by RNA polymerase II | ISO |
| GO:0000122 | negative regulation of transcription by RNA polymerase II | IMP |
| GO:0019827 | stem cell population maintenance | IGI |
| GO:0019827 | stem cell population maintenance | IMP |
| GO:0022008 | neurogenesis | IDA |
| GO:0030512 | negative regulation of transforming growth factor beta receptor signaling pathway | ISS |
| GO:0030512 | negative regulation of transforming growth factor beta receptor signaling pathway | IMP |
| GO:0030853 | negative regulation of granulocyte differentiation | ISS |
| GO:0030853 | negative regulation of granulocyte differentiation | ISO |
| GO:0035019 | somatic stem cell population maintenance | IDA |
| GO:0043457 | regulation of cellular respiration | IDA |
| GO:0043586 | tongue development | IMP |
| GO:0045892 | negative regulation of transcription, DNA-templated | ISS |
| GO:0045892 | negative regulation of transcription, DNA-templated | ISO |
| GO:0045892 | negative regulation of transcription, DNA-templated | IDA |
| GO:0045893 | positive regulation of transcription, DNA-templated | ISS |
| GO:0045893 | positive regulation of transcription, DNA-templated | ISO |
| GO:0045893 | positive regulation of transcription, DNA-templated | IDA |
| GO:0048468 | cell development | IC |
| GO:0050872 | white fat cell differentiation | IDA |
| GO:0050873 | brown fat cell differentiation | IMP |
| GO:0050873 | brown fat cell differentiation | IDA |
| GO:0051567 | histone H3-K9 methylation | IEA |
| GO:0060021 | roof of mouth development | IMP |
| GO:0070828 | heterochromatin organization | IMP |
| GO:0090336 | positive regulation of brown fat cell differentiation | IMP |
| GO:0120162 | positive regulation of cold-induced thermogenesis | IMP |
| GO ID | GO Term | Evidence |
|---|---|---|
| GO:0005634 | nucleus | ISO |
| GO:0005634 | nucleus | IDA |
| GO:0005654 | nucleoplasm | ISO |
| GO:0005829 | cytosol | ISO |
| GO:0016235 | aggresome | ISO |
| GO:0017053 | transcriptional repressor complex | IDA |
| Reactome ID | Reactome Term | Evidence |
|---|---|---|
| R-MMU-3214841 | PKMTs methylate histone lysines | IEA |
| R-MMU-3247509 | Chromatin modifying enzymes | IEA |
| R-MMU-4839726 | Chromatin organization | IEA |
| Keyword ID | Keyword Term |
|---|---|
| KW-0010 | Activator |
| KW-0025 | Alternative splicing |
| KW-0963 | Cytoplasm |
| KW-0221 | Differentiation |
| KW-0238 | DNA-binding |
| KW-0479 | Metal-binding |
| KW-0489 | Methyltransferase |
| KW-0539 | Nucleus |
| KW-1185 | Reference proteome |
| KW-0677 | Repeat |
| KW-0678 | Repressor |
| KW-0804 | Transcription |
| KW-0805 | Transcription regulation |
| KW-0808 | Transferase |
| KW-0862 | Zinc |
| KW-0863 | Zinc-finger |
| Pfam ID | Pfam Term |
|---|---|
| PF00096 | zf-C2H2 |