| Tag | Content |
|---|---|
| Uniprot ID | P54754; Q62214; Q91YS9; |
| Entrez ID | 13845 |
| Genbank protein ID | CAA88910.1; AAH53085.1; AAH14822.1; CAA53599.1; |
| Genbank nucleotide ID | NM_010143.1 |
| Ensembl protein ID | ENSMUSP00000006112 |
| Ensembl nucleotide ID | ENSMUSG00000005958 |
| Gene name | Ephrin type-B receptor 3 |
| Gene symbol | Ephb3 |
| Organism | Mus musculus |
| NCBI taxa ID | 10090 |
| Cleft type | CPO |
| Developmental stage | E12.5, E13.5, E14.5 |
| Data sources | Manually collected |
| Reference | 8947026 |
| Functional description | Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt. |
| Sequence | MAGARPPPGL LPLLAPLLLP LLLPAGCWAL EETLMDTKWV TSELAWTSHP ESGWEEVSGY 60 DEAMNPIRTY QVCNVRESSQ NNWLRTGFIW RREVQRVYVE LKFTVRDCNS IPNIPGSCKE 120 TFNLFYYEAD SDVASASSPF WMENPYVKVD TIAPDESFSR LDAGRVNTKV RSFGPLSKAG 180 FYLAFQDQGA CMSLISVRAF YKKCASTTAG FALFPETLTG AEPTSLVIAP GTCIANAVEV 240 SVPLKLYCNG DGEWMVPVGA CTCATGHEPA AKESQCRACP PGSYKAKQGE GPCLPCPPNS 300 RTTSPAASIC TCHNNFYRAD SDSADSACTT VPSPPRGVIS NVNETSLILE WSEPRDLGGR 360 DDLLYNVICK KCRGSSGAGG PATCSRCDDN VEFVPRQLGL TERRVHISHL LAHTRYTFEV 420 QAVNGVSGKS PLPPRYAAVN ITTNQAAPSE VPTLHLHSSS GSSLTLSWAP PERPNGVILD 480 YEMKYFEKSK GIASTVTSQK NSVQLDGLQP DARYVVQVRA RTVAGYGQYS HPAEFETTSE 540 RGSGAQQLQE QLPLIVGSTV AGFVFMVVVV VIALVCLRKQ RHGPDAEYTE KLQQYIAPGM 600 KVYIDPFTYE DPNEAVREFA KEIDVSCVKI EEVIGAGEFG EVCRGRLKLP GRREVFVAIK 660 TLKVGYTERQ RRDFLSEASI MGQFDHPNII RLEGVVTKSR PVMILTEFME NCALDSFLRL 720 NDGQFTVIQL VGMLRGIAAG MKYLSEMNYV HRDLAARNIL VNSNLVCKVS DFGLSRFLED 780 DPSDPTYTSS LGGKIPIRWT APEAIAYRKF TSASDVWSYG IVMWEVMSYG ERPYWDMSNQ 840 DVINAVEQDY RLPPPMDCPT ALHQLMLDCW VRDRNLRPKF SQIVNTLDKL IRNAASLKVT 900 ASAPSGMSQP LLDRTVPDYT TFTTVGDWLD AIKMGRYKES FVGAGFASFD LVAQMTAEDL 960 LRIGVTLAGH QKKILCSIQD MRLQMNQTLP VQV 993 |
Abbreviation :
CLO : cleft lip only. CPO : cleft palate only.
CLP : cleft lip and palate. CL/P : cleft lip with/without cleft palate.
For humans: CL/P, CLO, CPO, and CLP. For mice: CLO, CLP, and CPO.
| Relation | Gene symbol | Entrez ID | UniProt ID | Cleft type | Developmental stage | Species | Evidence | Details |
|---|---|---|---|---|---|---|---|---|
| 1:1 ortholog | EPHB3 | 540907 | F1ML02 | Bos taurus | Prediction | More>> | ||
| 1:1 ortholog | EPHB3 | A0A452DRK7 | Capra hircus | Prediction | More>> | |||
| 1:1 ortholog | EPHB3 | 2049 | P54753 | Homo sapiens | Prediction | More>> | ||
| 1:1 ortholog | Ephb3 | 13845 | P54754 | CPO | E12.5, E13.5, E14.5 | Mus musculus | Publication | More>> |
| 1:1 ortholog | EPHB3 | A0A2I3TIH1 | Pan troglodytes | Prediction | More>> | |||
| 1:1 ortholog | Ephb3 | 287989 | D3ZH39 | Rattus norvegicus | Prediction | More>> |
| GO ID | GO Term | Evidence |
|---|---|---|
| GO:0004714 | transmembrane receptor protein tyrosine kinase activity | IBA |
| GO:0005003 | ephrin receptor activity | ISS |
| GO:0005003 | ephrin receptor activity | ISO |
| GO:0005005 | transmembrane-ephrin receptor activity | TAS |
| GO:0005524 | ATP binding | IEA |
| GO:0008046 | axon guidance receptor activity | IDA |
| GO ID | GO Term | Evidence |
|---|---|---|
| GO:0001525 | angiogenesis | IMP |
| GO:0001655 | urogenital system development | IMP |
| GO:0007169 | transmembrane receptor protein tyrosine kinase signaling pathway | IBA |
| GO:0007275 | multicellular organism development | IBA |
| GO:0007411 | axon guidance | IMP |
| GO:0007411 | axon guidance | IDA |
| GO:0007413 | axonal fasciculation | IMP |
| GO:0016477 | cell migration | ISO |
| GO:0016477 | cell migration | IMP |
| GO:0018108 | peptidyl-tyrosine phosphorylation | IEA |
| GO:0021952 | central nervous system projection neuron axonogenesis | IDA |
| GO:0022038 | corpus callosum development | IMP |
| GO:0022407 | regulation of cell-cell adhesion | ISS |
| GO:0022407 | regulation of cell-cell adhesion | ISO |
| GO:0031290 | retinal ganglion cell axon guidance | IDA |
| GO:0033674 | positive regulation of kinase activity | IBA |
| GO:0034446 | substrate adhesion-dependent cell spreading | ISS |
| GO:0034446 | substrate adhesion-dependent cell spreading | ISO |
| GO:0043087 | regulation of GTPase activity | ISS |
| GO:0043087 | regulation of GTPase activity | ISO |
| GO:0046777 | protein autophosphorylation | ISS |
| GO:0046777 | protein autophosphorylation | ISO |
| GO:0048013 | ephrin receptor signaling pathway | ISS |
| GO:0048013 | ephrin receptor signaling pathway | ISO |
| GO:0048538 | thymus development | IMP |
| GO:0048546 | digestive tract morphogenesis | IMP |
| GO:0050770 | regulation of axonogenesis | IDA |
| GO:0051965 | positive regulation of synapse assembly | IMP |
| GO:0060021 | roof of mouth development | IMP |
| GO:0060996 | dendritic spine development | IMP |
| GO:0060997 | dendritic spine morphogenesis | IMP |
| GO ID | GO Term | Evidence |
|---|---|---|
| GO:0005887 | integral component of plasma membrane | ISS |
| GO:0005887 | integral component of plasma membrane | ISO |
| GO:0005887 | integral component of plasma membrane | TAS |
| GO:0005887 | integral component of plasma membrane | IBA |
| GO:0030425 | dendrite | IEA |
| GO:0043235 | receptor complex | IBA |
| Reactome ID | Reactome Term | Evidence |
|---|---|---|
| R-MMU-1266738 | Developmental Biology | IEA |
| R-MMU-2682334 | EPH-Ephrin signaling | IEA |
| R-MMU-3928662 | EPHB-mediated forward signaling | IEA |
| R-MMU-3928664 | Ephrin signaling | IEA |
| R-MMU-3928665 | EPH-ephrin mediated repulsion of cells | IEA |
| R-MMU-422475 | Axon guidance | IEA |
| Keyword ID | Keyword Term |
|---|---|
| KW-0037 | Angiogenesis |
| KW-0067 | ATP-binding |
| KW-1003 | Cell membrane |
| KW-0966 | Cell projection |
| KW-0217 | Developmental protein |
| KW-1015 | Disulfide bond |
| KW-0325 | Glycoprotein |
| KW-0418 | Kinase |
| KW-0472 | Membrane |
| KW-0524 | Neurogenesis |
| KW-0547 | Nucleotide-binding |
| KW-0597 | Phosphoprotein |
| KW-0675 | Receptor |
| KW-1185 | Reference proteome |
| KW-0677 | Repeat |
| KW-0732 | Signal |
| KW-0808 | Transferase |
| KW-0812 | Transmembrane |
| KW-1133 | Transmembrane helix |
| KW-0829 | Tyrosine-protein kinase |
| InterPro ID | InterPro Term |
|---|---|
| IPR027936 | Eph_TM |
| IPR034245 | EphB3_rcpt_lig-bd |
| IPR001090 | Ephrin_rcpt_lig-bd_dom |
| IPR003961 | FN3_dom |
| IPR036116 | FN3_sf |
| IPR008979 | Galactose-bd-like_sf |
| IPR013783 | Ig-like_fold |
| IPR011009 | Kinase-like_dom_sf |
| IPR000719 | Prot_kinase_dom |
| IPR017441 | Protein_kinase_ATP_BS |
| IPR001660 | SAM |
| IPR013761 | SAM/pointed_sf |
| IPR001245 | Ser-Thr/Tyr_kinase_cat_dom |
| IPR011641 | Tyr-kin_ephrin_A/B_rcpt-like |
| IPR008266 | Tyr_kinase_AS |
| IPR020635 | Tyr_kinase_cat_dom |
| IPR016257 | Tyr_kinase_ephrin_rcpt |
| IPR001426 | Tyr_kinase_rcpt_V_CS |