Gene: Ndst1

Basic information

Tag Content
Uniprot ID Q02353
Entrez ID 29633
Genbank protein ID AAA41701.1
Genbank nucleotide ID NM_024361.1; XM_017600928.1; XM_017600933.1; XM_017600929.1; XM_017600932.1; XM_017600931.1; XM_017600930.1;
Ensembl protein ID ENSRNOP00000025881
Ensembl nucleotide ID ENSRNOG00000019014
Gene name Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1
Gene symbol Ndst1
Organism Rattus norvegicus
NCBI taxa ID 10116
Cleft type
Developmental stage
Data sources Homology search
Reference
Functional description Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response. Required for the exosomal release of SDCBP, CD63 and syndecan.
Sequence
MPALACLRRL CRHLSPQAVL FLLFVFCLFS VFVSAYYLYG WNRGLEPSAD ASESDCGDPP 60
PVAPSRLLPI KPVQAVAPSR TDPLVLVFVE SLYSQLGQEV VAILESSRFK YRTEIAPGKG 120
DMPTLTDKGR GRFALIIYEN ILKYVNLDAW NRELLDKYCV AYGVGIIGFF KANENSLLSA 180
QLKGFPLFLH SNLGLKDCSI NPKSPLLYVT RPSEVEKGVL PGEDWTVFQS NHSTYEPVLL 240
AKTRSSESIP HLGADAGLHA ALHATVVQDL GLHDGIQRVL FGNNLNFWLH KLVFVDAVAF 300
LTGKRLSLPL DRYILVDIDD IFVGKEGTRM KVEDVKALFD TQNELRTHIP NFTFNLGYSG 360
KFFHTGTDAE DAGDDLLLSY VKEFWWFPHM WSHMQPHLFH NQSVLAEQMA LNKKFAVEHG 420
IPTDMGYAVA PHHSGVYPVH VQLYEAWKQV WNIRVTSTEE YPHLKPARYR RGFIHNGIMV 480
LPRQTCGLFT HTIFYNEYPG GSSELDKIIN GGELFLTVLL NPISVFMTHL SNYGNDRLGL 540
YTFKHLVRFL HSWTNLRLQT LPPVQLAQKY FQIFSEEKDP LWQDPCEDKR HKDIWSKEKT 600
CDRFPKLLII GPQKTGTTAL YLFLGMHPDL SSNYPSSETF EEIQFFNGHN YHKGIDWYME 660
FFPIPSNTTS DFYFEKSANY FDSEVAPRRA AALLPKAKVL TILINPADRA YSWYQHQRAH 720
DDPVALKYTF HEVITAGPDA SSKLRALQNR CLVPGWYATH IERWLSAFHA NQILVLDGKL 780
LRTEPAKVMD TVQKFLGVTS TVDYHKTLAF DPKKGFWCQL LEGGKTKCLG KSKGRKYPEM 840
DLDSRAFLKD YYRDHNIELS KLLYKMGQTL PTWLREDLQN TR 882

Abbreviation :
CLO : cleft lip only. CPO : cleft palate only. CLP : cleft lip and palate. CL/P : cleft lip with/without cleft palate.
For humans: CL/P, CLO, CPO, and CLP. For mice: CLO, CLP, and CPO.

Protein disorder information

Orthologous information

Relation Gene symbol Entrez ID UniProt ID Cleft type Developmental stage Species Evidence Details
1:1 orthologNDST1514172E1BCG5Bos taurusPredictionMore>>
1:1 orthologNDST1102174299A0A452EZE5Capra hircusPredictionMore>>
1:1 orthologNDST13340P52848Homo sapiensPredictionMore>>
1:1 orthologNdst115531Q3UHN9CPOMus musculusPublicationMore>>
1:1 orthologNDST1462194H2R584Pan troglodytesPredictionMore>>
1:1 orthologNDST1M3V841Sus scrofaPredictionMore>>
1:1 orthologNDST1100344304G1T1F7Oryctolagus cuniculusPredictionMore>>
1:1 orthologNdst129633Q02353Rattus norvegicusPredictionMore>>
1:1 orthologndst1b570459A0A0R4IR84Danio rerioPredictionMore>>

Gene ontology

GO ID GO Term
GO:0005794 Golgi apparatus
GO:0000139 Golgi membrane
GO:0016021 integral component of membrane
GO:0015016 [heparan sulfate]-glucosamine N-sulfotransferase activity
GO:0019213 deacetylase activity
GO:0042328 heparan sulfate N-acetylglucosaminyltransferase activity
GO:0102140 heparan sulfate N-deacetylase activity
GO:0008146 sulfotransferase activity
GO:0009887 animal organ morphogenesis
GO:0035904 aorta development
GO:0003279 cardiac septum development
GO:0060976 coronary vasculature development
GO:0048702 embryonic neurocranium morphogenesis
GO:0048703 embryonic viscerocranium morphogenesis
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0030900 forebrain development
GO:0030203 glycosaminoglycan metabolic process
GO:0007507 heart development
GO:0015012 heparan sulfate proteoglycan biosynthetic process
GO:0015014 heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process
GO:0030210 heparin biosynthetic process
GO:0006954 inflammatory response
GO:0030901 midbrain development
GO:0000271 polysaccharide biosynthetic process
GO:0043410 positive regulation of MAPK cascade
GO:0045880 positive regulation of smoothened signaling pathway
GO:0006477 protein sulfation
GO:0007585 respiratory gaseous exchange by respiratory system

Functional annotations

Keywords

Keyword ID Keyword Term
KW-0903 Direct protein sequencing
KW-1015 Disulfide bond
KW-0325 Glycoprotein
KW-0333 Golgi apparatus
KW-0378 Hydrolase
KW-0395 Inflammatory response
KW-0472 Membrane
KW-0511 Multifunctional enzyme
KW-1185 Reference proteome
KW-0735 Signal-anchor
KW-0808 Transferase
KW-0812 Transmembrane
KW-1133 Transmembrane helix

Interpro

InterPro ID InterPro Term
IPR021930 Heparan_SO4_deacetylase
IPR037359 NST/OST
IPR027417 P-loop_NTPase
IPR000863 Sulfotransferase_dom

PROSITE

PROSITE ID PROSITE Term

Pfam

Pfam ID Pfam Term
PF12062 HSNSD
PF00685 Sulfotransfer_1