| Tag | Content |
|---|---|
| Uniprot ID | Q498R1; D3ZDG8; |
| Entrez ID | 290947 |
| Genbank protein ID | EDL87600.1; AAI00108.1; |
| Genbank nucleotide ID | NM_001039003.1; XM_006227813.3; |
| Ensembl protein ID | ENSRNOP00000058505; ENSRNOP00000024041; |
| Ensembl nucleotide ID | ENSRNOG00000017826 |
| Gene name | Methionine synthase reductase |
| Gene symbol | Mtrr |
| Organism | Rattus norvegicus |
| NCBI taxa ID | 10116 |
| Cleft type | |
| Developmental stage | |
| Data sources | Homology search |
| Reference | |
| Functional description | Key enzyme in methionine and folate homeostasis responsible for the reactivation of methionine synthase (MTR/MS) activity by catalyzing the reductive methylation of MTR-bound cob(II)alamin. Cobalamin (vitamin B12) forms a complex with MTR to serve as an intermediary in methyl transfer reactions that cycles between MTR-bound methylcob(III)alamin and MTR bound-cob(I)alamin forms, and occasional oxidative escape of the cob(I)alamin intermediate during the catalytic cycle leads to the inactive cob(II)alamin species. The processing of cobalamin in the cytosol occurs in a multiprotein complex composed of at least MMACHC, MMADHC, MTRR and MTR which may contribute to shuttle safely and efficiently cobalamin towards MTR in order to produce methionine (By similarity). Also necessary for the utilization of methyl groups from the folate cycle, thereby affecting transgenerational epigenetic inheritance (By similarity). Also acts as a molecular chaperone for methionine synthase by stabilizing apoMTR and incorporating methylcob(III)alamin into apoMTR to form the holoenzyme. Also serves as an aquacobalamin reductase by reducing aquacobalamin to cob(II)alamin in the presence of NADPH, and this reduction leads to stimulation of the conversion of apoMTR and aquacobalamin to MTR holoenzyme (By similarity). |
| Sequence | MRRFLLLYAT QRGQAKAIAE EISEQALSHG FSADLHCVSE SEKYDLKTET GPLVMVVSTT 60 GTGDPPDTAR KFVKEIHNKT LPTDFFAHLW YGLLGLGDSE YTYFCNGGKV IDKRLQELGA 120 QHFYDTGHAD DCVGLELVVE PWIDGLWPAL TKHFKSLGGQ EDMSDSDTLA QASDAPLSMA 180 MKPELLHIQS QVELLSLEDV GKRDSELQEQ NETNKNQPSR IEDFDSSLVN SVPPLSQSSL 240 SIPAVSPEYL EVYLQESLGQ DENQASVPPS VDPIFQVPIS KAVELTTNDA IKTTLLLELD 300 ISKVEFSHQP GDSFNVICPN SGSEVEDLLQ RLQLADKQAH RVILKIKMDT KKKGASLPQH 360 VPEGSSLQFI FTWCLEIRAV PKKAFLRALS DYTSDATEKR RLQELCSKQG AADYNRFIRD 420 ASVCLLDLLL TFPSCQPPLN LLLEHLPKLQ PRPYSCASSS LLHPDKLHFV FNIVELPSNT 480 TAASLRKGVC TGWLATLVAP FLQPNTEVLT ADHSDALAPE ILISPRATNS FHLPDDLSAP 540 IIMVGPGTGV APFVGFLQHR EKLQEQHPDG NFGAMWLFFG CRHKDRDYLF REELRHFLKT 600 GVLTHLKVSF SRDAAPEEEE EAPAKYVQDN LQHHSQQVAR TLLQENGYIY VCGDAKNMAK 660 DVHDALVEII SKEAGVDKLE AMKTLATLKQ EKRYLQDIWS |
Abbreviation :
CLO : cleft lip only. CPO : cleft palate only.
CLP : cleft lip and palate. CL/P : cleft lip with/without cleft palate.
For humans: CL/P, CLO, CPO, and CLP. For mice: CLO, CLP, and CPO.
| Relation | Gene symbol | Entrez ID | UniProt ID | Cleft type | Developmental stage | Species | Evidence | Details |
|---|---|---|---|---|---|---|---|---|
| 1:1 ortholog | MTRR | 507991 | F1MGP5 | Bos taurus | Prediction | More>> | ||
| 1:1 ortholog | MTRR | 102171193 | A0A452F9C4 | Capra hircus | Prediction | More>> | ||
| 1:1 ortholog | MTRR | 4552 | Q9UBK8 | CPO,CL/P | Homo sapiens | Publication | More>> | |
| 1:1 ortholog | Mtrr | 210009 | A0A0R4J0G9 | Mus musculus | Prediction | More>> | ||
| 1:1 ortholog | MTRR | 461717 | H2QQM3 | Pan troglodytes | Prediction | More>> | ||
| 1:1 ortholog | MTRR | F1S0T1 | Sus scrofa | Prediction | More>> | |||
| 1:1 ortholog | Mtrr | 290947 | Q498R1 | Rattus norvegicus | Prediction | More>> | ||
| 1:1 ortholog | mtrr | 560667 | F1RE28 | Danio rerio | Prediction | More>> |
| GO ID | GO Term |
|---|---|
| GO:0005829 | cytosol |
| GO:0030586 | [methionine synthase] reductase activity |
| GO:0050444 | aquacobalamin reductase (NADPH) activity |
| GO:0071949 | FAD binding |
| GO:0050660 | flavin adenine dinucleotide binding |
| GO:0010181 | FMN binding |
| GO:0070402 | NADPH binding |
| GO:0003958 | NADPH-hemoprotein reductase activity |
| GO:0016491 | oxidoreductase activity |
| GO:0016709 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen |
| GO:0016723 | oxidoreductase activity, oxidizing metal ions, NAD or NADP as acceptor |
| GO:0006306 | DNA methylation |
| GO:0046655 | folic acid metabolic process |
| GO:0043418 | homocysteine catabolic process |
| GO:0050667 | homocysteine metabolic process |
| GO:0009086 | methionine biosynthetic process |
| GO:1904042 | negative regulation of cystathionine beta-synthase activity |
| GO:0055114 | oxidation-reduction process |
| GO:0033353 | S-adenosylmethionine cycle |
| InterPro ID | InterPro Term |
|---|---|
| IPR003097 | CysJ-like_FAD-binding |
| IPR017927 | FAD-bd_FR_type |
| IPR001094 | Flavdoxin-like |
| IPR008254 | Flavodoxin/NO_synth |
| IPR001709 | Flavoprot_Pyr_Nucl_cyt_Rdtase |
| IPR029039 | Flavoprotein-like_sf |
| IPR039261 | FNR_nucleotide-bd |
| IPR023173 | NADPH_Cyt_P450_Rdtase_alpha |
| IPR001433 | OxRdtase_FAD/NAD-bd |
| IPR017938 | Riboflavin_synthase-like_b-brl |