| Tag | Content |
|---|---|
| Uniprot ID | Q8K5C0; Q80UZ5; |
| Entrez ID | 252973 |
| Genbank protein ID | AAM22619.1; AAH55035.1; AAH42575.1; |
| Genbank nucleotide ID | NM_026496.4 |
| Ensembl protein ID | ENSMUSP00000022895 |
| Ensembl nucleotide ID | ENSMUSG00000022286 |
| Gene name | Grainyhead-like protein 2 homolog |
| Gene symbol | Grhl2 |
| Organism | Mus musculus |
| NCBI taxa ID | 10090 |
| Cleft type | CPO |
| Developmental stage | |
| Data sources | Manually collected |
| Reference | 21515572; 26177923; 20654612; |
| Functional description | Transcription factor playing an important role in primary neurulation and in epithelial development. Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' acting as an activator and repressor on distinct target genes (PubMed:22696678). During embryogenesis, plays unique and cooperative roles with GRHL3 in establishing distinct zones of primary neurulation. Essential for closure 3 (rostral end of the forebrain), functions cooperatively with GRHL3 in closure 2 (forebrain/midbrain boundary) and posterior neuropore closure (PubMed:20654612). Regulates epithelial morphogenesis acting as a target gene-associated transcriptional activator of apical junctional complex components. Up-regulates of CLDN3 and CLDN4, as well as of RAB25, which increases the CLDN4 protein and its localization at tight junctions (PubMed:22696678). Comprises an essential component of the transcriptional machinery that establishes appropriate expression levels of CLDN4 and CDH1 in different types of epithelia (PubMed:20978075). Exhibits functional redundancy with GRHL3 in epidermal morphogenetic events such as eyelid fusion and epidermal wound repair (PubMed:21081122). In lung, forms a regulatory loop with NKX2-1 that coordinates lung epithelial cell morphogenesis and differentiation (PubMed:22955271). In keratinocytes, plays a role in telomerase activation during cellular proliferation, regulates TERT expression by binding to TERT promoter region and inhibiting DNA methylation at the 5'-CpG island, possibly by interfering with DNMT1 enzyme activity. In addition, impairs keratinocyte differentiation and epidermal function by inhibiting the expression of genes clustered at the epidermal differentiation complex (EDC) as well as GRHL1 and GRHL3 through epigenetic mechanisms (By similarity). |
| Sequence | MSQESDNNKR LVALVPMPSD PPFNTRRAYT SEDEAWKSYL ENPLTAATKA MMSINGDEDS 60 AAALGLLYDY YKVPRDKRLL SVSKASDSQE DQDKRNCLGT SEAQINLSGG ENRVQVLKTV 120 PVNLCLSQDH MENSKREQYS VSITESSAVI PVSGITVVKA EDFTPVFMAP PVHYPRADSE 180 EQRVVIFEQT QYDLPSIASH SSYLKDDQRS TPDSTYSESF KDGASEKFRS TSVGADEYTY 240 DQTGSGTFQY TLEATKSLRQ KQGEGPMTYL NKGQFYAITL SETGDNKCFR HPISKVRSVV 300 MVVFSEDKNR DEQLKYWKYW HSRQHTAKQR VLDIADYKES FNTIGNIEEI AYNAVSFTWD 360 VNEEAKIFIT VNCLSTDFSS QKGVKGLPLM IQIDTYSYNN RSNKPIHRAY CQIKVFCDKG 420 AERKIRDEER KQNRKKGKGQ ASQAQCNNSS DGKMAAIPLQ KKSDITYFKT MPDLHSQPVL 480 FIPDVHFANL QRTGQVYYNT DDEREGSSVL VKRMFRPMEE EFGPTPSKQI KEENVKRVLL 540 YVRKENDDVF DALMLKSPTV KGLMEALSEK YGLPVEKITK LYKKSKKGIL VNMDDNIIEH 600 YSNEDTFILN MESMVEGFKI TLMEI 625 |
Abbreviation :
CLO : cleft lip only. CPO : cleft palate only.
CLP : cleft lip and palate. CL/P : cleft lip with/without cleft palate.
For humans: CL/P, CLO, CPO, and CLP. For mice: CLO, CLP, and CPO.
