DegronMD

Uniprot ID: P11388

Source protein: DNA topoisomerase 2-alpha

Source gene: TOP2A

Class: DEG_SPOP_SBC_1

Location: 1389:1393

E3 ligase: SPOP

DEGRON score: 0.9972

ASA_SCORE: 84.08

COIL: 0.9406

CONS_SCORE: 0.2141

DSS_SCORE: 0.6216

HELIX: 0.0228

FCONS_SCORE: 1.0283

RIG_SCORE: 0.5677

Degron related mutations and impact score

Mutation	Sample	Cancer	Dataset	Degron	Degron class	Impact score
p.E1380K	TCGA-3C-AALI	BRCA	TCGA	PLSSS	DEG_SPOP_SBC_1	0.0833
p.E1380K	DO219589	BRCA	ICGC	PLSSS	DEG_SPOP_SBC_1	0.0833
p.E1380K	SIDM00946	T-Lymphoblastic Leukemia	GDSC	PLSSS	DEG_SPOP_SBC_1	0.0833
p.E1380K	ACH-000981	Leukemia	CCLE	PLSSS	DEG_SPOP_SBC_1	0.0833
p.E1380K	DND-41	haematopoietic_and_lymphoid_tissue	COSMIC	PLSSS	DEG_SPOP_SBC_1	0.0833
p.K1385N	TCGA-EE-A29L	SKCM	TCGA	PLSSS	DEG_SPOP_SBC_1	0.6667
p.K1385N	DO37835	SKCM	ICGC	PLSSS	DEG_SPOP_SBC_1	0.6667
p.P1394T	TCGA-FS-A1ZB	SKCM	TCGA	PLSSS	DEG_SPOP_SBC_1	0.3056
p.P1394T	DO37886	SKCM	ICGC	PLSSS	DEG_SPOP_SBC_1	0.3056
p.D1382H	TCGA-JW-A5VL	CESC	TCGA	PLSSS	DEG_SPOP_SBC_1	0.1389
p.D1382H	DO49976	CESC	ICGC	PLSSS	DEG_SPOP_SBC_1	0.1389
p.D1382H	DO234314	ESAD	ICGC	PLSSS	DEG_SPOP_SBC_1	0.1389
p.P1395T	DO231793	LINC	ICGC	PLSSS	DEG_SPOP_SBC_1	0.2778
p.P1394R	ACH-001956	Bone Cancer	CCLE	PLSSS	DEG_SPOP_SBC_1	0.3056

Drug responses associated with TOP2A mutations

Sample	Cancer	Mutation (In Degron?)	Dataset	Drug	IC50
SIDM00918	OV\|ovary	p.S1392* (Yes)	GDSC	Etoposide	79.2721
SIDM00190	SKCM\|melanoma	p.V255I (No)	GDSC	Etoposide	4.873
SIDM00951	LAML\|acute_myeloid_leukaemia	p.R675* (No)	GDSC	Etoposide	0.4151
SIDM00145	ALL\|lymphoblastic_leukemia	p.S1491F (No)	GDSC	Etoposide	0.2463
SIDM00145	ALL\|lymphoblastic_leukemia	p.R268H (No)	GDSC	Etoposide	0.2463
SIDM00388	UNCLASSIFIED\|B_cell_leukemia	p.R1435fs*13 (No)	GDSC	Etoposide	6.9919
SIDM00654	SCLC\|lung_small_cell_carcinoma	p.Q1453fs*3 (No)	GDSC	Etoposide	0.5619
SIDM00104	MESO\|mesothelioma	p.K1110E (No)	GDSC	Etoposide	2.9539
SIDM00956	ESCA\|oesophagus	p.K893R (No)	GDSC	Etoposide	7.1187
SIDM01007	ALL\|lymphoblastic_leukemia	p.V1506M (No)	GDSC	Etoposide	21.8046
SIDM01007	ALL\|lymphoblastic_leukemia	p.P1457L (No)	GDSC	Etoposide	21.8046
SIDM01007	ALL\|lymphoblastic_leukemia	p.E626K (No)	GDSC	Etoposide	21.8046
SIDM01007	ALL\|lymphoblastic_leukemia	p.A588S (No)	GDSC	Etoposide	21.8046
SIDM01007	ALL\|lymphoblastic_leukemia	p.D222N (No)	GDSC	Etoposide	21.8046
SIDM00666	LGG\|glioma	p.T930I (No)	GDSC	Etoposide	0.976
SIDM00844	UNCLASSIFIED\|Burkitt_lymphoma	p.G1192R (No)	GDSC	Etoposide	9.0519
SIDM00690	HNSC\|head_and_neck	p.G1197A (No)	GDSC	Etoposide	0.6063
SIDM00938	BRCA\|breast	p.S1449C (No)	GDSC	Etoposide	0.6923
SIDM01090	COREAD\|large_intestine	p.K1489E (No)	GDSC	Etoposide	0.5276
SIDM01090	COREAD\|large_intestine	p.Y613C (No)	GDSC	Etoposide	0.5276
SIDM01259	ALL\|lymphoblastic_leukemia	p.T1205fs*19 (No)	GDSC	Etoposide	8.5228
SIDM01020	MM\|myeloma	p.A318P (No)	GDSC	Etoposide	4.4968
SIDM00252	UNCLASSIFIED\|biliary_tract	p.R635H (No)	GDSC	Etoposide	33.2918
SIDM00252	UNCLASSIFIED\|biliary_tract	p.T530A (No)	GDSC	Etoposide	33.2918
SIDM00251	STAD\|stomach	p.M102fs*14 (No)	GDSC	Etoposide	47.1095
SIDM00792	SCLC\|lung_small_cell_carcinoma	p.N774I (No)	GDSC	Etoposide	10.4314
SIDM00438	UNCLASSIFIED\|B_cell_leukemia	p.T1205fs*19 (No)	GDSC	Etoposide	21.6344
SIDM00946	ALL\|T_cell_leukemia	p.E1380K (Yes)	GDSC	Etoposide	0.7725
SIDM00946	ALL\|T_cell_leukemia	p.T357I (No)	GDSC	Etoposide	0.7725
SIDM00946	ALL\|T_cell_leukemia	p.L231P (No)	GDSC	Etoposide	0.7725
SIDM00602	STAD\|stomach	p.T1205fs*19 (No)	GDSC	Etoposide	4.9204
SIDM00313	UNCLASSIFIED\|Hodgkin_lymphoma	p.G1216C (No)	GDSC	Etoposide	11.9554
SIDM00794	UNCLASSIFIED\|skin_other	p.Q35R (No)	GDSC	Etoposide	2.1041
SIDM00783	COREAD\|large_intestine	p.Q657fs*4 (No)	GDSC	Etoposide	0.8065
SIDM00599	UNCLASSIFIED\|osteosarcoma	p.V776L (No)	GDSC	Etoposide	1.043
SIDM01096	COREAD\|large_intestine	p.K1110E (No)	GDSC	Etoposide	1.888
SIDM00699	SCLC\|lung_small_cell_carcinoma	p.P643T (No)	GDSC	Etoposide	7.4048
SIDM00363	LAML\|acute_myeloid_leukaemia	p.L944S (No)	GDSC	Etoposide	95.1444
SIDM00886	DLBC\|B_cell_lymphoma	p.S1428P (No)	GDSC	Etoposide	6.8141
SIDM00739	LUAD\|lung_NSCLC_adenocarcinoma	p.K1196I (No)	GDSC	Etoposide	1.8655
SIDM00263	UNCLASSIFIED\|ewings_sarcoma	p.G1198R (No)	GDSC	Etoposide	38.1681
SIDM00257	NB\|neuroblastoma	p.P786H (No)	GDSC	Etoposide	1.7136
SIDM01090	COREAD\|large_intestine	p.T620fs*7 (No)	GDSC	Etoposide	0.5276
SIDM00143	SCLC\|lung_small_cell_carcinoma	p.D152Y (No)	GDSC	Etoposide	2.0328
SIDM00427	UNCLASSIFIED\|rhabdomyosarcoma	p.N774I (No)	GDSC	Etoposide	0.1621
SIDM00789	COREAD\|large_intestine	p.L326S (No)	GDSC	Etoposide	8.7985
SIDM00534	COREAD\|large_intestine	p.S1425F (No)	GDSC	Etoposide	11.7804
SIDM00534	COREAD\|large_intestine	p.S527* (No)	GDSC	Etoposide	11.7804
SIDM00680	COREAD\|large_intestine	p.T1205fs*19 (No)	GDSC	Etoposide	1.3046
SIDM00963	BRCA\|breast	p.V1097L (No)	GDSC	Etoposide	113.841
SIDM00412	UNCLASSIFIED\|lymphoid_neoplasm_other	p.R1435fs*13 (No)	GDSC	Etoposide	2.9138
SIDM00663	SKCM\|melanoma	p.S1337L (No)	GDSC	Etoposide	6.6804
SIDM01116	UNCLASSIFIED\|lung_NSCLC_large_cell	p.V1001L (No)	GDSC	Etoposide	1.7035
SIDM00683	PRAD\|prostate	p.R1435fs*13 (No)	GDSC	Etoposide	10.7002
SIDM00142	UCEC\|endometrium	p.R1435fs*13 (No)	GDSC	Etoposide	11.6305
SIDM01080	HNSC\|head_and_neck	p.N774I (No)	GDSC	Etoposide	0.8558
SIDM00040	STAD\|stomach	p.V68I (No)	GDSC	Etoposide	4.4022
SIDM01113	UNCLASSIFIED\|soft_tissue_other	p.V364L (No)	GDSC	Etoposide	0.0552
SIDM00339	LUAD\|lung_NSCLC_adenocarcinoma	p.S1337L (No)	GDSC	Etoposide	1.9732
SIDM01259	ALL\|lymphoblastic_leukemia	p.A903D (No)	GDSC	Etoposide	8.5228
SIDM01027	ESCA\|oesophagus	p.P716R (No)	GDSC	Etoposide	1.9148
SIDM00423	DLBC\|B_cell_lymphoma	p.G1197E (No)	GDSC	Etoposide	0.3217
SIDM00374	DLBC\|B_cell_lymphoma	p.D213G (No)	GDSC	Etoposide	1.7691
SIDM00054	PAAD\|pancreas	p.S1361F (No)	GDSC	Etoposide	0.6636
SIDM00531	PAAD\|pancreas	p.H290Q (No)	GDSC	Etoposide	1.1689
SIDM01010	UNCLASSIFIED\|anaplastic_large_cell_lymphoma	p.R1435G (No)	GDSC	Etoposide	4.1415
SIDM00118	COREAD\|large_intestine	p.Q1206H (No)	GDSC	Etoposide	1.0944
SIDM00661	DLBC\|B_cell_lymphoma	p.R1435fs*13 (No)	GDSC	Etoposide	16.3821

Uniprot ID	Source protein	Degron class	Location	Hit	E3 ligase	Degron score	Detail
O00763	Acetyl-CoA carboxylase 2	DEG_SPOP_SBC_1	103:107	PLSSS	SPOP	0.9873	More...
O43516	WAS/WASL-interacting protein family member 1	DEG_SPOP_SBC_1	239:243	PLSSS	SPOP	0.9749	More...
O60825	6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2	DEG_SPOP_SBC_1	469:473	PLSSS	SPOP	0.9968	More...
O75140	GATOR complex protein DEPDC5	DEG_SPOP_SBC_1	829:833	PLSSS	SPOP	0.9934	More...
O75152	Zinc finger CCCH domain-containing protein 11A	DEG_SPOP_SBC_1	685:689	PLSSS	SPOP	0.9688	More...
P04637	Cellular tumor antigen p53	DEG_SPOP_SBC_1	92:96	PLSSS	SPOP	0.9977	More...
P11388	DNA topoisomerase 2-alpha	DEG_SPOP_SBC_1	1389:1393	PLSSS	SPOP	0.9972	More...
P14316	Interferon regulatory factor 2	DEG_SPOP_SBC_1	312:316	PLSSS	SPOP	0.9872	More...
P19484	Transcription factor EB	DEG_SPOP_SBC_1	185:189	PLSSS	SPOP	0.9793	More...
P55198	Protein AF-17	DEG_SPOP_SBC_1	574:578	PLSSS	SPOP	0.9943	More...
Q00613	Heat shock factor protein 1	DEG_SPOP_SBC_1	288:292	PLSSS	SPOP	0.9368	More...
Q13470	Non-receptor tyrosine-protein kinase TNK1	DEG_SPOP_SBC_1	541:545	PLSSS	SPOP	0.9727	More...
Q15911	Zinc finger homeobox protein 3	DEG_SPOP_SBC_1	3634:3638	PLSSS	SPOP	0.9557	More...
Q2TBE0	CWF19-like protein 2	DEG_SPOP_SBC_1	388:392	PLSSS	SPOP	0.9997	More...
Q6AI39	BRD4-interacting chromatin-remodeling complex-associated protein-like	DEG_SPOP_SBC_1	77:81	PLSSS	SPOP	0.9962	More...
Q6P0Q8	Microtubule-associated serine/threonine-protein kinase 2	DEG_SPOP_SBC_1	114:118	PLSSS	SPOP	0.9937	More...
Q6T4R5	Nance-Horan syndrome protein	DEG_SPOP_SBC_1	1473:1477	PLSSS	SPOP	0.9884	More...
Q6ZN55	Zinc finger protein 574	DEG_SPOP_SBC_1	118:122	PLSSS	SPOP	0.9413	More...
Q6ZQX7	Protein LIAT1	DEG_SPOP_SBC_1	113:117	PLSSS	SPOP	0.961	More...
Q6ZRS2	Helicase SRCAP	DEG_SPOP_SBC_1	1434:1438	PLSSS	SPOP	0.9226	More...
Q86UP3	Zinc finger homeobox protein 4	DEG_SPOP_SBC_1	509:513	PLSSS	SPOP	0.9784	More...
Q8IVF2	Protein AHNAK2	DEG_SPOP_SBC_1	4735:4739	PLSSS	SPOP	0.6704	More...
Q8IZ21	Phosphatase and actin regulator 4	DEG_SPOP_SBC_1	174:178	PLSSS	SPOP	0.636	More...
Q8N684	Cleavage and polyadenylation specificity factor subunit 7	DEG_SPOP_SBC_1	247:251	PLSSS	SPOP	0.9926	More...
Q8NDV7	Trinucleotide repeat-containing gene 6A protein	DEG_SPOP_SBC_1	1354:1358	PLSSS	SPOP	0.959	More...
Q8TAX7	Mucin-7	DEG_SPOP_SBC_1	248:252	PLSSS	SPOP	0.8247	More...
Q92993	Histone acetyltransferase KAT5	DEG_SPOP_SBC_1	133:137	PLSSS	SPOP	0.9685	More...
Q96B97	SH3 domain-containing kinase-binding protein 1	DEG_SPOP_SBC_1	573:577	PLSSS	SPOP	0.9968	More...
Q96MU7	YTH domain-containing protein 1	DEG_SPOP_SBC_1	73:77	PLSSS	SPOP	0.9922	More...
Q96P47	Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3	DEG_SPOP_SBC_1	478:482	PLSSS	SPOP	0.779	More...
Q9NS37	CREB/ATF bZIP transcription factor	DEG_SPOP_SBC_1	129:133	PLSSS	SPOP	0.9398	More...
Q9NSC2	Sal-like protein 1	DEG_SPOP_SBC_1	1112:1116	PLSSS	SPOP	0.9707	More...
Q9NXE4	Sphingomyelin phosphodiesterase 4	DEG_SPOP_SBC_1	244:248	PLSSS	SPOP	0.5515	More...
Q9NZM4	BRD4-interacting chromatin-remodeling complex-associated protein	DEG_SPOP_SBC_1	1226:1230	PLSSS	SPOP	0.982	More...
Q9P0L1	Zinc finger protein with KRAB and SCAN domains 7	DEG_SPOP_SBC_1	365:369	PLSSS	SPOP	0.9637	More...
Q9P218	Collagen alpha-1	DEG_SPOP_SBC_1	615:619	PLSSS	SPOP	0.9976	More...
Q9P2B4	CTTNBP2 N-terminal-like protein	DEG_SPOP_SBC_1	405:409	PLSSS	SPOP	0.9931	More...
Q9P2P6	StAR-related lipid transfer protein 9	DEG_SPOP_SBC_1	1734:1738	PLSSS	SPOP	0.9258	More...
Q9UBC0	Hepatocyte nuclear factor 6	DEG_SPOP_SBC_1	181:185	PLSSS	SPOP	0.5617	More...
Q9UIU6	Homeobox protein SIX4	DEG_SPOP_SBC_1	311:315	PLSSS	SPOP	0.9014	More...
Q9ULK0	Glutamate receptor ionotropic, delta-1	DEG_SPOP_SBC_1	954:958	PLSSS	SPOP	0.9853	More...
Q9ULT8	E3 ubiquitin-protein ligase HECTD1	DEG_SPOP_SBC_1	1507:1511	PLSSS	SPOP	0.9967	More...
Q9UPX0	Protein turtle homolog B	DEG_SPOP_SBC_1	914:918	PLSSS	SPOP	0.9928	More...
Q9Y4D2	Diacylglycerol lipase-alpha	DEG_SPOP_SBC_1	993:997	PLSSS	SPOP	0.7658	More...
Q9Y5Y9	Sodium channel protein type 10 subunit alpha	DEG_SPOP_SBC_1	952:956	PLSSS	SPOP	0.9773	More...
Q8IZF5	Adhesion G-protein coupled receptor F3	DEG_SPOP_SBC_1	69:73	PLSSS	SPOP	0.8794	More...
Q96PE2	Rho guanine nucleotide exchange factor 17	DEG_SPOP_SBC_1	670:674	PLSSS	SPOP	0.9963	More...
Q9H2A3	Neurogenin-2	DEG_SPOP_SBC_1	32:36	PLSSS	SPOP	0.972	More...
Q9UN71	Protocadherin gamma-B4	DEG_SPOP_SBC_1	429:433	PLSSS	SPOP	0.7211	More...
Q9Y5F8	Protocadherin gamma-B7	DEG_SPOP_SBC_1	431:435	PLSSS	SPOP	0.7253	More...
Q9Y5G0	Protocadherin gamma-B5	DEG_SPOP_SBC_1	427:431	PLSSS	SPOP	0.8178	More...
Q9Y5G2	Protocadherin gamma-B2	DEG_SPOP_SBC_1	431:435	PLSSS	SPOP	0.6125	More...
Q6ZMS4	Zinc finger protein 852	DEG_SPOP_SBC_1	141:145	PLSSS	SPOP	0.745	More...
Q6ZV89	SH2 domain-containing protein 5	DEG_SPOP_SBC_1	171:175	PLSSS	SPOP	0.9938	More...
Q8N9Z9	Lamin tail domain-containing protein 1	DEG_SPOP_SBC_1	35:39	PLSSS	SPOP	0.8742	More...
Q96N46	Tetratricopeptide repeat protein 14	DEG_SPOP_SBC_1	578:582	PLSSS	SPOP	0.8795	More...
Q96RY5	Protein cramped-like	DEG_SPOP_SBC_1	1056:1060	PLSSS	SPOP	0.9467	More...
Q86XN7	Proline and serine-rich protein 1	DEG_SPOP_SBC_1	780:784	PLSSS	SPOP	0.9515	More...
Q9H6K5	Proline-rich protein 36	DEG_SPOP_SBC_1	298:302	PLSSS	SPOP	0.9654	More...

DegronMD: protein-targeted degradation, mutation and drug response to degrons

Degron: PLSSS

Degron related mutations and impact score

Drug responses associated with TOP2A mutations

All proteins holding degron of PLSSS